Journal: Tumour Biology

Article Title: Identification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue

doi: 10.1007/s13277-016-5275-8

Figure Lengend Snippet: Manual dissection of FFPE colon tissue. Representative image shows non-cancer and cancer regions before ( a and b , respectively) and after ( c and d , respectively) dissection. Sections were stained with hematoxylin

Article Snippet: Total RNA was extracted from FFPE CRC tissue with the RNeasy FFPE Kit (QIAGEN, Valencia, CA, USA) and from CRC cell lines with the GenElute Mammalian Total RNA Miniprep Kit (Sigma). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit for FFPE tissue and the High Capacity cDNA Reverse Transcription kit for the CRC cell line according to the manufacturer’s protocols (Life Technologies Japan, Tokyo, Japan).

Techniques: Dissection, Staining

Journal: Tumour Biology

Article Title: Identification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue

doi: 10.1007/s13277-016-5275-8

Figure Lengend Snippet: Venn map of proteins identified from FFPE colorectal cancer tissue. We identified 204 proteins in the cancer regions and 142 in the non-cancer regions

Article Snippet: Total RNA was extracted from FFPE CRC tissue with the RNeasy FFPE Kit (QIAGEN, Valencia, CA, USA) and from CRC cell lines with the GenElute Mammalian Total RNA Miniprep Kit (Sigma). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit for FFPE tissue and the High Capacity cDNA Reverse Transcription kit for the CRC cell line according to the manufacturer’s protocols (Life Technologies Japan, Tokyo, Japan).

Techniques:

Journal: Tumour Biology

Article Title: Identification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue

doi: 10.1007/s13277-016-5275-8

Figure Lengend Snippet: Semiquantitative comparison of proteins identified in FFPE colon tissue. Rsc and normalized spectral abundance factor (NSAF) values calculated for the proteins identified ( x -axis). Comparison of protein expression in cancer versus non-cancer tissue. Proteins highly expressed in either cancer or non-cancer regions were plotted near the right or left side of the x -axis

Article Snippet: Total RNA was extracted from FFPE CRC tissue with the RNeasy FFPE Kit (QIAGEN, Valencia, CA, USA) and from CRC cell lines with the GenElute Mammalian Total RNA Miniprep Kit (Sigma). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit for FFPE tissue and the High Capacity cDNA Reverse Transcription kit for the CRC cell line according to the manufacturer’s protocols (Life Technologies Japan, Tokyo, Japan).

Techniques: Comparison, Expressing